| Description : |
Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including animal organ development; generation of neurons; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; cranium; early conceptus; and female reproductive system. Orthologous to human LIF (LIF interleukin 6 family cytokine). |
| Source : |
E. coli |
| Species : |
Mouse |
| Bio-activity : |
Mouse LIF activity is assessed by its ability to induce differentiation of M1 myeloid leukemia cells. The specific activity is 1 × 10^6 units/ml and 1 × 10^7 units/ml, where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL of medium. |
| Molecular Mass : |
20 kDa |
| Protein Length : |
181 amino acid |
| Endotoxin : |
< 0.01 ng/μg cytokine as determined by the LAL assay |
| Purity : |
> 98% by SDS-PAGE |
| Usage : |
1 ml of mouse LIF with the specific activity of 1x106 units/ml is sufficient to treat 1 L of embryonic stem cell culture media. 1 mL of mouse LIF with the specific activity of 1x107 units/ml is sufficient to treat 10 L of embryonic stem cell culture media. |
| Storage : |
It is stable for 6 to 12 months at 2-4 centigrade in original formulation. For extended storage, it is recommended to store in working aliquots at -20 to -80 centigrade. Freeze-thaw should be avoided as it can result in loss of activity. |
| Storage Buffer : |
1×phosphate-buffered saline with 0.02% Tween-20. Sterile filtered through a 0.2 micron filter. |
| Shipping : |
This product is shipped at ambient temperature with cold pack. |
