Active Recombinant Mouse Lif Protein, Pre-aliquoted

• Cat.No.: Lif-27M
• Product Overview: Recombinant Mouse Lif Protein, Pro25 - Phe203, was expressed in E. coli.

Specification

• Description: Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including animal organ development; generation of neurons; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; cranium; early conceptus; and female reproductive system. Orthologous to human LIF (LIF interleukin 6 family cytokine).
• Source: E. coli
• Species: Mouse
• Bio-activity: Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 is 0.1-0.6 ng/mL.
• Molecular Mass: Predicted Molecular Mass:20 kDa
  SDS-PAGE: 20 kDa, reducing conditions
• Protein length: Pro25-Phe203
• N-terminal Sequence Analysis: Pro25
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 0.2-1 mg/mL in sterile PBS. For immediate use, simply roll Pre-aliquoted directly into cell culture medium.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Lif leukemia inhibitory factor [ Mus musculus (house mouse) ]
• Official Symbol: Lif
• Synonyms: Lif; leukemia inhibitory factor; leukemia inhibitory factor; d factor; differentiation-stimulating factor; myeloid leukaemia inhibitory factor
• Gene ID: 16878
• mRNA Refseq: NM_008501
• Protein Refseq: NP_032527
• UniProt ID: P09056