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Recombinant Mouse Ephrin A1, His-tagged

Cat. No.: Efna1-425M
Product Overview: Recombinant Mouse Efna1is expressed in Murine myeloma cell line, which has a molecule weight of 46.8kDa. Asp19 ­ Ser182.
Description: Ephrin­A1, also knownas B61 and LERK­1, is a member of the Ephrin­A family of GPI­anchored ligandsthat bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin­Aligands are structurally related to the extracellular domains of the transmembraneEphrin­B ligands. It can also be released as a soluble molecule. The mature 21­25 kDa mouse Ephrin­A1 shares 85% and 94% aa sequence identity with human andrat Ephrin­A1, respectively. Ephrin­A1 is widely expressed on endothelial andepithelial cells, particularly in the lung, intestine, liver, and skin. It inhibitstumor cell proliferation and migration but also supports tumor growth by promotingangiogenesis. Soluble Ephrin­A1 additionally promotes neuronal survival and neuriteextension.
Source: Murine myeloma cell line,NS0­derived
Form: Lyophilized from a 0.2μm filteredsolution in PBS.
N-terminal Sequence: Asp19
Molecular Weight: 46.8 kDa
Activity: Measured by its bindingability in a functional ELISA. Immobilized rmEphA2/Fc Chimera at 2 µg/mL (100µL/well) can bind rmEphrin­A1 Fc Chimera with a line range of 0.16 ­ 10 ng/mL.
Purity: >90%, by SDS-PAGEunder reducing conditions and visualized by silver stain.
Endotoxin Level: <1.0 EU per 1 μg of the proteinby the LAL method.
Reconstitution: Reconstitute at 100 μg/mL in PBS.
Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
OfficialSymbol: Efna1
Gene Name: Efna1 ephrin A1 [ Mus musculus]
Synonyms: Efna1; ephrin A1; B61;Efl1; Epl1; Eplg1; Lerk1; LERK-1; AI325262; ephrin-A1; immediate early responseprotein B61; PH-related receptor tyrosine kinase ligand 1; EPLG1; TNFAIP4; ECKLG;EPH-related receptor tyrosine kinase ligand 1; Tumor necrosis factor alpha-inducedprotein 4; OTTHUMP00000033242; OTTHUMP00000033271
Gene ID: 13636
mRNA Refseq: NM_001162425
Protein Refseq: NP_001155897
UniProt ID: P52793
Pathway: Axon guidance
Function: ephrin receptor binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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