"SOST" Related Products


Recombinant Human SOST, His-tagged

Cat. No.: SOST-925H
Product Overview: Recombinant humanSOST with an N­terminal 7­His tag was expressed in mouse myeloma cell, 22.5 kDa.
Description: Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
Source: NS0cells
Species: Human
N­terminal Sequence Analysis: His
Activity: Measured by its ability to inhibit Wnt­3a­induced alkaline phosphatase production by MC3T3-E1mouse preosteoblast cells. The ED50 for this effect is typically 1.5-6μg/mL in the presence of 3ng/mL Recombinant Mouse Wnt­3a. Measured by its ability to inhibit BMP­6­induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. 30µg/mL of rhSOST will antagonize hBMP­6 induction of alkaline phosphatase in MC3T3E1 cells by 50­80%.
Structure / Form: Monomer
Predicted MolecularMass: 22.5 kDa
Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method
Purity: > 95% as determined by SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°Cas supplied. 1 month, 2 to 8°Cunder sterile conditions after reconstitution. 3 months, -20 to -70°Cunder sterile conditions after reconstitution.
Gene Name: SOST sclerosteosis [Homo sapiens]
Official Symbol: SOST
Synonyms: SOST; VBCH; sclerostin; sclerosteosis
Gene ID: 50964
mRNA Refseq: NM_025237
Protein Refseq: NP_079513
MIM: 605740
UniProt ID: Q9BQB4
Chromosome Location: 17q11.2
Function: heparin binding; protein binding; transcription factor binding

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