Recombinant Human IL1F7

• Cat.No.: IL1F7-318H
• Product Overview: Recombinant Human IL-1F7 (Lys27-Asp192) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 167 amino acids and having a molecular mass of 18.6 kDa.

Specification

• Cat. No.: IL1F7-318H
• Description: Human interleukin family 1, member 7 (hIL-1F7 / FIL1-zeta) is a member of the interleukin 1 cytokine family. Five alternatively spliced transcript variants encoding distinct isoforms have been reported with distinct expression profiles. The longest IL-1F7 transcript, referred to as IL1F7b or IL1F7 isoform 1, encodes a 218 a.a. residues proprotein containing a 45 a.a. propeptide which is cleaved to generate mature protein. IL-1F7b binds to IL-18 Rα with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds interleukin 18 binding protein (IL-18BP), an inhibitory binding protein of interleukin 18 (IL-18), and subsequently forms a complex with IL-18 receptor beta subunit, and through which it inhibits the activity of IL-18.
• Biological Activity: As measured by its binding ability in a functional ELISA, immobilized rHuIL-1F7 at 1 μg/ml (100 μl/well) can bind rhIL-18 R/Fc Chimera with a linear range of 0.015~1 μg/mL.
• Purity: Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Asn-Leu-Asn.
• Endotoxin: Less than 0.1ng/µg (1 IEU/µg) determined by LAL test.
• Form: The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4.
• Reconstitution: It is recommended to quick spin followed by reconstitution of rHuIL-1F7 in PBS to a concentration no less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Lyophilized rHuIL-1F5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-1F7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: IL1F7 interleukin 1 family, member 7 (zeta) [ Homo sapiens ]
• Official Symbol: IL1F7
• Synonyms: IL1F7; interleukin 1 family, member 7 (zeta); FIL1; FIL1Z; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA); interleukin-1 family member 7 ; FIL1 zeta; IL-1 zeta; IL-1X protein; OTTHUMP00000162032; OTTHUMP00000162033; OTTHUMP00000162034; OTTHUMP00000162035; OTTHUMP00000162036; interleukin-1 zeta; interleukin 1, zeta; interleukin-1 homolog 4; interleukin-1 superfamily z; interleukin 1 family member 7; interleukin-1-related protein; IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL1F7 (canonical product IL-1F7b); Interleukin-1 zeta; Interleukin-1 homolog 4; Interleukin-1-related protein; IL-1X
• Gene ID: 27178
• mRNA Refseq: NM_014439
• Protein Refseq: NP_055254
• MIM: 605510
• UniProt ID: Q9NZH6
• Chromosome Location: 2q12-q14.1
• Function: cytokine activity; interleukin-1 receptor antagonist activity; interleukin-1 receptor binding; protein binding