| Cat. No. : |
LEP-3626H |
| Description : |
Leptin encoded by obese gene is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signalling pathways involving OB-R in hypothalamus. Animal models have revealed the influence of Leptin in reducing body weight and regulating blood glucose level. When mutations are introduced in obese gene, mice with impaired function of leptin are massively obese and in high risk of diabetes. Leptin deficiency reduces metablic rate. Leptin deficient mice are less active and with lower body temperature than normal animals. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin consists of 167 amino acid residue including a 21 residue signal sequence and 146 residue mature protein sequence. |
| Biological Activity : |
The ED50 of rHuLeptin as determined by dose-dependent cell proliferation assay using human Leptin R transfected BaF3 mouse proB cells was found to be approximately 0.42ng/ml. |
| Purity : |
Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Amino-Acid Sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Val-Pro-Ile-Aln-Lys. |
| Endotoxin : |
Less than 0.1ng/μg (1.0EU/μg) of rHuGalectin-8 as determined by LAL test. |
| Form : |
The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM PBS, pH 7.4. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized rHuGalectin-8 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage : |
Lyophilized rHuLeptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuLeptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
