| Cat. No. : |
Egf-038E |
| Description : |
Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGFμ, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparinbinding EGFlike growth factor (HBEGF), epigen, and the neuregulins (NRG)1 through 6. Members of the EGF family share a structural motif, the EGFlike domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture. |
| Source : |
E. coli |
| Nterminal Sequence Analysi : |
Met |
| Molecular Mass : |
6.2 kDa |
| Activity : |
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 20 100 pg/mL. |
| Endotoxin Leve : |
<1.0 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>97%, by SDSPAGE under reducing conditions and visualized by silver stain. |
| Formulation : |
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. See Certificate of Analysis for details. |
| Reconstitution : |
Reconstitute at 0.1 mg/mL in sterile PBS. |
| Shipping : |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage : |
Use a manual defrost freezer and avoid repeated freezethaw cycles. 1. 12 months from date of receipt, 20 to 70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, 20 to 70 °C under sterile conditions after reconstitution. |
