Recombinant Mouse Egf (977-1029aa), Met tagged

Cat. No.: Egf-038E
Product Overview: Recombinant Mouse Egf (Asn977 ­ Arg1029, with an N­terminal Met ) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.
Description: Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF­μ, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin­binding EGF­like growth factor (HB­EGF), epigen, and the neuregulins (NRG)­1 through ­6. Members of the EGF family share a structural motif, the EGF­like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
Source: E. coli
N­terminal Sequence Analysi: Met
Molecular Mass: 6.2 kDa
Activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 20 ­ 100 pg/mL.
Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. See Certificate of Analysis for details.
Reconstitution: Reconstitute at 0.1 mg/mL in sterile PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
Gene Name: Egf epidermal growth factor [ Mus musculus ]
Official Symbol: Egf
Synonyms: Egf; epidermal growth factor; AI790464; pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF)
Gene ID: 13645
mRNA Refseq: NM_010113
Protein Refseq: NP_034243
Chromosome Location: 3 G3; 3 65.2 cM
Pathway: Delta-Notch Signaling Pathway; EGFR1 Signaling Pathway; ESC Pluripotency Pathways; ErbB signaling pathway; MAPK signaling pathway; TGF Beta Signaling Pathway
Function: calcium ion binding; epidermal growth factor receptor binding; growth factor activity; protein binding

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