Recombinant Mouse Egf protein

• Cat.No.: Egf-05H
• Product Overview: Recombinant Mouse Egf protein(P01132) was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Form: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10^7 IU/mg.
• Molecular Mass: Approximately 6.0 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids, including 3 intramolecular disulfide-bonds.
• AASequence: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
• Endotoxin: Less than 1 EU/μg of rMuEGF as determined by LAL method.
• Purity: > 97 %
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.

Gene Information

• Gene Name: Egf epidermal growth factor [ Mus musculus ]
• Official Symbol: Egf
• Synonyms: EGF; epidermal growth factor; pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); AI790464
• Gene ID: 13645
• mRNA Refseq: NM_010113
• Protein Refseq: NP_034243

Citation

Mucus mediated protection against acute colitis in adiponectin deficient mice

Journal: Journal of Inflammation (London, England)    PubMed ID: 25949213    Data: 2015/4/29

Authors: Kamaljeet Kaur, Arpit Saxena, Raja Fayad

Article Snippet:These mice received 2% dextran sodium sulfate (DSS) (MW 36,000-50,000 MP Biochemicals) in drinking water for 5 days followed by normal drinking water for next 5 days, which constitutes a single cycle of DSS administration and represents the induction of acute inflammation in the gut.These mice received 2% dextran sodium sulfate (DSS) (MW 36,000-50,000 MP Biochemicals) in drinking water for 5 days followed by normal drinking water for next 5 days, which constitutes a single cycle of DSS administration and represents the induction of acute inflammation in the gut.. Treatment groups were administered either 1.5 mg/kg bodyweight of recombinant murine APN (Creative BioMart, New York) or the same amount of PBS intraperitoneally depending on the treatment group every alternate day during the 10-day period.. Clinical scores were based on weight, diarrhea and fecal hemoccult on a quantifiable scale of 12 as follows: (i) a score of 1, 2, 3, or 4 was assigned for 0-5%, 6-10%, 11-15%, 16-20% and more than 20% weight loss respectively, (ii) a score of 0, 2 or 4 was assigned for well-formed fecal pellets, pasty and semi-formed fecal pellets and liquid stools that adhere to the anus, respectively, and, (iii) a score of 0, 2 or 4 was assigned to absence of blood in stools, positive hemoccult and gross bleeding respectively.Clinical scores were based on weight, diarrhea and fecal hemoccult on a quantifiable scale of 12 as follows: (i) a score of 1, 2, 3, or 4 was assigned for 0-5%, 6-10%, 11-15%, 16-20% and more than 20% weight loss respectively, (ii) a score of 0, 2 or 4..