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| Cat. No.: | FGF1-564H |
| Product Overview: | Recombinant Human Fibroblast Growth Factor-acidic (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15,967 Daltons. |
| Description: | Single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. It binds to heparin, which potentiates its biological activity and protects it from proteolysis. |
| Species: | Human |
| Source: | E.coli |
| Form: | Recombinant FGF-acidic was lyophilized from a sterile solution containing 10mM NaP pH=7.5 and 75 mM NaCl. |
| Purity: | Purity Greater than 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Bio-activity: | Recombinant Human Fibroblast Growth Factor-alpha is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is <1.0 ng/ml. |
| Physical Appearance: | Sterile Filtered white lyophilized (freeze-dried) powder. |
| Solubility: | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions. |
| Purity Greater than 97% as determined by: | Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Protein Content determined by: | UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
| Endotoxin Level: | Endotoxin level is <0.01ng/ug or <0.1EU/ug. |
| Amino acid sequence: | MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D |
| Storage: | Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
| Pathways: | MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton |
| Gene Name: | FGF1 fibroblast growth factor 1 (acidic) [ Homo sapiens ] |
| Official Symbol: | FGF1 |
| Synonyms: | FGF1; fibroblast growth factor 1 (acidic); AFGF; ECGF; FGFA; ECGFA; ECGFB; HBGF1; GLIO703; FGF-alpha; ECGF-beta; Acidic fibroblast growth factor; OTTHUMP00000066031; heparin-binding growth factor 1; endothelial cell growth factor, beta; endothelial cell growth factor, alpha; Beta-endothelial cell growth factor; OTTHUMP00000066028; OTTHUMP00000174675; OTTHUMP00000066030 |
| Gene ID: | 2246 |
| mRNA Refseq: | NM_000800 |
| Protein Refseq: | NP_000791 |
| MIM: | 131220 |
| UniProt ID: | P05230 |
| Chromosome Location: | 5q31 |
| Function: | growth factor activity; heparin binding; protein binding |
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