Expand
Expand
| Cat. No.: | IL15-483H |
| Product Overview: | Recombinant human IL-15 produced in E.coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12,773.5 Daltons. |
| Description: | Interleukin 15 (IL-15) is a pleiotropic pro-inflammatory cytokine that promotes activation of T cells, neutrophils and macrophages, and is critical to dendritic cell function in several model systems. IL-15 has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes. |
| Species: | Human |
| Source: | E.coli |
| Form: | Recombinant IL-15 was lyophilized from a concentrated (1mg/ml) solution with 5mM Tris pH 8.0. |
| Purity: | Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Bio-activity: | Recombinant IL-15 is fully biologically active when compared to standards. The ED50, as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 cells, was found to be < 0.5 ng/ml. |
| Physical Appearance: | Sterile Filtered white lyophilized (freeze-dried) powder. |
| Solubility: | It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Purity Greater than 98% as determined by: | Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Protein Content determined by: | UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
| Endotoxin Level: | Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
| Amino acid sequence: | MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS |
| Storage: | Lyophilized product is very stable at -20 °C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added to the sample for long term storage. |
| Pathways: | Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway |
| Gene Name: | IL15 interleukin 15 [ Homo sapiens ] |
| Official Symbol: | IL15 |
| Synonyms: | IL15; interleukin 15; IL-15; MGC9721; OTTHUMP00000164617 |
| Gene ID: | 3600 |
| mRNA Refseq: | NM_000585 |
| Protein Refseq: | NP_000576 |
| MIM: | 600554 |
| UniProt ID: | P40933 |
| Chromosome Location: | 4q31 |
| Function: | cytokine activity; cytokine receptor binding; protein binding; signal transducer activity |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Download Datasheet
See All IL15 Products
Download Datasheet