| Cat. No.: | IL15-483H |
| Product Overview: | Recombinant human IL-15 produced in E.coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12,773.5 Daltons. |
| Description: | Interleukin 15 (IL-15) is a pleiotropic pro-inflammatory cytokine that promotes activation of T cells, neutrophils and macrophages, and is critical to dendritic cell function in several model systems. IL-15 has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes. |
| Species: | Human |
| Source: | E.coli |
| Form: | Recombinant IL-15 was lyophilized from a concentrated (1mg/ml) solution with 5mM Tris pH 8.0. |
| Purity: | Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Bio-activity: | Recombinant IL-15 is fully biologically active when compared to standards. The ED50, as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 cells, was found to be < 0.5 ng/ml. |
| Physical Appearance: | Sterile Filtered white lyophilized (freeze-dried) powder. |
| Solubility: | It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Purity Greater than 98% as determined by: | Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Protein Content determined by: | UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
| Endotoxin Level: | Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
| Amino acid sequence: | MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS |
| Storage: | Lyophilized product is very stable at -20 °C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added to the sample for long term storage. |
| Pathways: | Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway |
| Gene Name: | IL15 interleukin 15 [ Homo sapiens ] |
| Official Symbol: | IL15 |
| Synonyms: | IL15; interleukin 15; IL-15; MGC9721; OTTHUMP00000164617 |
| Gene ID: | 3600 |
| mRNA Refseq: | NM_000585 |
| Protein Refseq: | NP_000576 |
| MIM: | 600554 |
| UniProt ID: | P40933 |
| Chromosome Location: | 4q31 |
| Function: | cytokine activity; cytokine receptor binding; protein binding; signal transducer activity |
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