Recombinant Human Interleukin 15 IL15-483H

Recombinant Human Interleukin 15

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Recombinant Human Interleukin 15

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Cat. No. : IL15-483H
Product Overview : Recombinant human IL-15 produced in E.coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12,773.5 Daltons.
Description : Interleukin 15 (IL-15) is a pleiotropic pro-inflammatory cytokine that promotes activation of T cells, neutrophils and macrophages, and is critical to dendritic cell function in several model systems. IL-15 has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.
Species : Human
Source : E.coli
Form : Recombinant IL-15 was lyophilized from a concentrated (1mg/ml) solution with 5mM Tris pH 8.0.
Purity : Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity : Recombinant IL-15 is fully biologically active when compared to standards. The ED50, as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 cells, was found to be < 0.5 ng/ml.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 98% as determined by : Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by : UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level : Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence : MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS
Storage : Lyophilized product is very stable at -20 °C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added to the sample for long term storage.
Pathways : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
Renderingbased on PDB 2Z3Q. :

Gene Name : IL15 interleukin 15 [ Homo sapiens ]
Official Symbol : IL15
Synonyms : IL15; interleukin 15; IL-15; MGC9721; OTTHUMP00000164617
Gene ID : 3600
mRNA Refseq : NM_000585
Protein Refseq : NP_000576
MIM : 600554
UniProt ID : P40933
Chromosome Location : 4q31
Function : cytokine activity; cytokine receptor binding; protein binding; signal transducer activity

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