Cat. No.: |
IL15-483H |
Product Overview: |
Recombinant human IL-15 produced in E.coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12,773.5 Daltons. |
Description: |
Interleukin 15 (IL-15) is a pleiotropic pro-inflammatory cytokine that promotes activation of T cells, neutrophils and macrophages, and is critical to dendritic cell function in several model systems. IL-15 has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes. |
Species: |
Human |
Source: |
E.coli |
Form: |
Recombinant IL-15 was lyophilized from a concentrated (1mg/ml) solution with 5mM Tris pH 8.0. |
Purity: |
Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
Bio-activity: |
Recombinant IL-15 is fully biologically active when compared to standards. The ED50, as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 cells, was found to be < 0.5 ng/ml. |
Physical Appearance: |
Sterile Filtered white lyophilized (freeze-dried) powder. |
Solubility: |
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
Purity Greater than 98% as determined by: |
Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
Protein Content determined by: |
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
Endotoxin Level: |
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
Amino acid sequence: |
MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS |
Storage: |
Lyophilized product is very stable at -20 °C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added to the sample for long term storage. |
Pathways: |
Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway |