| Cat. No. : |
IL33-322H |
| Description : |
IL33 is a cytokine belonging to the IL-1 superfamily. IL33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. This cytokine was previously named NF-HEV "nuclear factor (NF) in high endothelial venules, (HEVs) since it was originally identified in these specialized cells. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-μB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. The induction of type 2 cytokines by IL33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL33. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20 mM TRIS, 50 mM NaCl, Ph 7.5 |
| Research topic : |
Energy metabolism and body weight regulation |
| Purity : |
>95% |
| Reconstitution : |
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Applications : |
Western blotting |
| Quality Control Test : |
BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein. |
| Sequences of amino acids : |
MKHHHHHHAS SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET |
| Storage : |
Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C. |
