Species : |
Human |
Source : |
Sf9 Cells |
Tag : |
GST |
Protein Length : |
532-1153 a.a. |
Description : |
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein that functions as a histone acetyltransferase. EP300 regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. EP300 binding is a highly accurate process and can be used for identifying enhancers and their associated activities. EP300 is also useful in the study of the role of tissue-specific enhancers in human biology and diseases on a genome wide scale. Defects in EP300 gene are a cause of Rubinstein-Taybi syndrome that play a important role in epithelial cancer and act as a classic tumor suppressor gene. |
Applications : |
Western Blot |
Molecular Weight : |
120 kDa |
Expression System : |
Sf9 insect cells using baculovirus |
Form : |
Recombinant protein stored in 50 mM Tris-HCl, ph 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. |
Purity : |
> 80 % |
Concentration : |
0.1 ug/ul |
Sequences : |
532-1153; contains the catalytic domain |
Storage : |
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Pathways : |
ATF-2 transcription factor network; Adherens junction; Androgen Receptor Signaling Pathway; C-MYB transcription factor network; Cell cycle; Delta-Notch Signaling Pathway; Diabetes pathways; Direct p53 effectors; E2F transcription factor network; Energy Metabolism; FOXA1 transcription factor network; FOXM1 transcription factor network; Influenza A; Melanogenesis; Regulation of Wnt-mediated beta catenin signaling and target gene transcription |