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Recombinant Mouse Il1b protein

Cat.No. : Il1b-04M
Product Overview : Recombinant Mouse Il1b protein was expressed in Escherichia coli.
Description : Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. The mature mouse IL1β shares 90 % amino acid sequence identity with cotton rat and rat and 65 %-78 % identity with canine, human and rhesus IL1β.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 10⁸ IU/mg.
Molecular Mass : Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Protein length : 152
AA Sequence : VPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS
Endotoxin : Less than 1 EU/µg of rMuIL-1β as determined by LAL method.
Purity : >96% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
SDS-PAGE of Il1b-04M :

Gene Name : Il1b
Official Symbol : Il1b
Synonyms : IL1B; interleukin 1 beta; interleukin-1 beta; IL-1 beta; Il-1b; IL-1beta;
Gene ID : 16176
mRNA Refseq : NM_008361
Protein Refseq : NP_032387
UniProt ID : P10749

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