Active Recombinant Mouse Il1b protein, His-tagged

• Cat.No.: Il1b-7238M
• Product Overview: Recombinant Mouse Il1b protein(Val118-Ser269), fused with C-terminal His tag, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 118-269 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Mouse IL1B at 10 μg/mL (100 μL/well) can bind Human IL1R2 with a linear range of 0.03125-0.8036 μg/mL. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 42.1-168.4 pg/mL, corresponding to a specific activity of 5.94×10^6-2.37×10^7 units/mg.
• Molecular Mass: 18.23 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Il1b interleukin 1 beta [ Mus musculus ]
• Official Symbol: Il1b
• Synonyms: IL1B; interleukin 1 beta; interleukin-1 beta; IL-1 beta; Il-1b; IL-1beta
• Gene ID: 16176
• mRNA Refseq: NM_008361
• Protein Refseq: NP_032387