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Recombinant Mouse Il1b protein

Cat.No. : Il1b-04M
Product Overview : Recombinant Mouse Il1b protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
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Description : Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. The mature mouse IL1β shares 90 % amino acid sequence identity with cotton rat and rat and 65 %-78 % identity with canine, human and rhesus IL1β.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 10⁸ IU/mg.
Molecular Mass : Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Protein length : 152
AA Sequence : VPIRQLHYRLRDEQQKSLVLSDPYE LKALHLNGQNINQQVIFSMSFVQGE PSNDKIPVALGLKGKNLYLSCVMKD GTPTLQLESVDPKQYPKKKMEKRFV FNKIEVKSKVEFESAEFPNWYISTS QAEHKPVFLGNNSGQDIIDFTMESV SS
Endotoxin : Less than 1 EU/µg of rMuIL-1β as determined by LAL method.
Purity : >96% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Il1b
Official Symbol : Il1b
Synonyms : IL1B; interleukin 1 beta; interleukin-1 beta; IL-1 beta; Il-1b; IL-1beta;
Gene ID : 16176
mRNA Refseq : NM_008361
Protein Refseq : NP_032387
UniProt ID : P10749

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How does IL-1β initiate inflammasome assembly, and which experimental techniques unravel this intricate process? 07/30/2022

Inflammasome assembly initiated by IL-1β is deciphered using techniques like co-immunoprecipitation, proximity ligation assays, and super-resolution microscopy.

What are the specific mechanisms underlying IL-1β's priming of immune cells, and how can flow cytometry and gene expression analyses decipher these molecular pathways? 07/08/2021

IL-1β's priming of immune cells is dissected through flow cytometry, RNA sequencing, and chromatin immunoprecipitation, revealing its epigenetic and transcriptional effects.

How do post-translational modifications regulate IL-1β's biological activity, and how can mass spectrometry-based phosphoproteomics provide insights into these modifications? 06/06/2020

Mass spectrometry-based phosphoproteomics deciphers IL-1β's post-translational modifications, revealing regulatory sites crucial for its activation and signaling.

How can advanced bioinformatics tools be applied to multi-omics data to comprehensively understand the intricate roles of IL-1β in health and disease? 04/16/2020

Bioinformatics tools integrate multi-omics data to provide a comprehensive understanding of IL-1β's roles, revealing its intricate contributions to health and disease.

What is the significance of IL-1β's epigenetic regulation in chronic inflammatory diseases, and how can ChIP-seq and DNA methylation profiling uncover these regulatory elements? 11/01/2019

ChIP-seq and DNA methylation profiling uncover IL-1β's epigenetic impact on target genes, shedding light on its role in shaping chronic inflammatory states.

How does IL-1β interact with its downstream effectors to induce the production of pro-inflammatory cytokines, and how can dual-luciferase reporter assays reveal these intricate interactions? 03/31/2019

IL-1β's interactions with downstream effectors are unveiled using dual-luciferase reporter assays, surface plasmon resonance, and co-crystallization studies.

What are the complex feedback loops between IL-1β and other cytokines, and how can systems biology approaches like network analysis dissect these dynamic interactions? 01/24/2019

Network analysis integrates omics data to unravel IL-1β's intricate cross-talk with other cytokines, mapping out complex regulatory loops in inflammation.

What is the role of IL-1β in modulating the tumor microenvironment, and how can advanced imaging techniques like intravital microscopy elucidate its impact on immune cell dynamics? 08/08/2017

IL-1β's impact on the tumor microenvironment is studied using intravital microscopy, 3D culture models, and single-cell RNA sequencing to capture dynamic cell behavior.

What are the mechanisms underlying IL-1β's non-canonical signaling pathways, and how can CRISPR-Cas9 screening identify key mediators of these alternative pathways? 04/18/2017

CRISPR-Cas9 screening identifies IL-1β's non-canonical signaling mediators through functional genomics, revealing alternative pathways in cellular responses.

How does IL-1β's signaling adapt to different cellular contexts, and how can single-cell RNA sequencing uncover cell-specific responses to this cytokine? 01/03/2017

Single-cell RNA sequencing unveils IL-1β's context-specific responses, exposing cell type-specific signaling and gene expression patterns.

Customer Reviews (3)

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Reviews
11/08/2021

    Effective in inflammation studies.

    04/23/2021

      High specificity.

      06/30/2020

        Suitable for cytokine assays.

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