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Active Recombinant Mouse Sparc, His-tagged

Cat.No. : Sparc-4030M
Product Overview : Recombinant Mouse Sparc (Ala18-Ile302) fused with 10­His tag at C­terminal, was expressed in Mouse NSO Cells.
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Description : SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM­40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N­terminal acidic region that binds calcium, a follistatin domain containing Kazal­like sequences, and a C­terminal extracellular calcium (EC) binding domain with two EF­hand motifs. Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N­glycosylation site are opposite this face. SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC shows context­specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis. SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper­binding sequence KGHK. Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation. Stabilin­1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation. Mature mouse SPARC shows 97%, 92%, 92%, 92% and 83% aa identity with rat, human, dog, cow and chick SPARC, respectively.
Source : Mammalian cells
Species : Mouse
Tag : His
Predicted N Terminal : Ala18
Formulation : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. The ED50 for this effect is typically 0.6 - 2.4 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : 34 kDa
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS.
Gene Name : Sparc secreted acidic cysteine rich glycoprotein [ Mus musculus(house mouse) ]
Official Symbol : Sparc
Synonyms : Sparc; secreted acidic cysteine rich glycoprotein; BM-40; AA517111; SPARC; ON; osteonectin; basement-membrane protein 40; secreted protein acidic and rich in cysteine
Gene ID : 20692
mRNA Refseq : NM_009242
Protein Refseq : NP_033268
UniProt ID : P07214
Chromosome Location : 11 B1; 11 33.04 cM
Pathway : Hemostasis; Platelet activation, signaling and aggregation; Platelet degranulation; Senescence and Autophagy
Function : calcium ion binding; extracellular matrix binding; metal ion binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How is the SPARC protein linked to cancer? 04/18/2023

SPARC is associated with cancer progression, as it can influence tumor growth, invasion, and angiogenesis. Its expression levels in tumors are often studied for prognostic and diagnostic purposes.

Can SPARC be a potential therapeutic target for cancer treatment? 12/08/2022

Yes, SPARC is being explored as a therapeutic target for cancer. Researchers are investigating ways to modulate its activity to hinder tumor progression or enhance the efficacy of existing treatments.

In what ways can SPARC expression be detected and measured in clinical settings? 04/25/2021

SPARC expression can be detected through techniques like immunohistochemistry, Western blotting, and molecular assays. These methods help assess SPARC levels in tissues or blood samples.

How does SPARC contribute to tissue repair and regeneration? 03/04/2021

SPARC plays a role in tissue repair by influencing cell-matrix interactions and modulating the extracellular environment. Its involvement in wound healing and tissue regeneration makes it a subject of interest in regenerative medicine.

Are there any ongoing clinical trials targeting SPARC for therapeutic interventions? 03/28/2019

Yes, there are clinical trials exploring the potential of targeting SPARC in various diseases, including cancer. These trials aim to evaluate the safety and efficacy of SPARC-modulating agents.

Customer Reviews (3)

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Reviews
07/18/2021

    The SPARC protein finds great utility in protein electron microscopy structure analysis, consistently delivering exceptional results.

    02/09/2017

      Its remarkable sensitivity and specificity make it a valuable tool for accurate and robust data analysis, bolstering the credibility of research outcomes.

      07/10/2016

        Notably, it exhibits exceptional performance in ELISA assays, providing reliable and precise results in the detection and quantification of target molecules.

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