Recombinant Rat Sparc protein, His-tagged

• Cat.No.: Sparc-7699R
• Product Overview: Recombinant Rat Sparc aa. (Ala18~Ile301 (Accession # P16975)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.6kDa
• Protein length: Ala18~Ile301 (Accession # P16975)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: Sparc secreted protein acidic and cysteine rich [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Sparc
• Synonyms: BM-40; ON; Secreted acidic cystein-rich glycoprotein (osteonectin); basement-membrane protein 40; osteonectin; secreted acidic cysteine rich glycoprotein; secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC
• Gene ID: 24791
• mRNA Refseq: NM_012656.1
• Protein Refseq: NP_036788.1
• UniProt ID: P16975