Recombinant Human PCSK9, HIS/HA/AVI-tagged

• Cat.No.: PCSK9-153H
• Product Overview: Recombinant Human Proprotein Convertase Subtilisin/Kexin Type 9/PCSK9 (biotinylated with His tag) is produced by our mammalian expression system. The target protein is expressed with sequence (Q31-Q692) of Human PCSK9 fused with a HIS/HA/AVI tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&HA&His
• Protein Length: 31-692 a.a.
• Description: Human Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) is a secretory subtilase belonging to the proteinase K subfamily. PCSK9 is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the ER , the pro domain and mature chain secrete together through noncovalent interactions. PCSK9 binds with low-density lipoprotein receptor (LDLR) and plays a major regulatory role in cholesterol homeostasis. Inhibition of PCSK9 function by preventing PCSK9/LDLR interaction is currently being explored as a means of lowering cholesterol levels. PCSK9 also binds to apolipoprotein receptor 2 (ApoER2), and play a role in the neural development.
• Endotoxin: Less than 0.1 ng/μg (1 IEU/μg).
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Gene Information

• Gene Name: PCSK9 proprotein convertase subtilisin/kexin type 9 [ Homo sapiens ]
• Official Symbol: PCSK9
• Synonyms: PCSK9; proprotein convertase subtilisin/kexin type 9; HCHOLA3, hypercholesterolemia, autosomal dominant 3; FH3; NARC 1; NARC1; NARC-1; HCHOLA3
• Gene ID: 353175
• MIM: 607786
• UniProt ID: Q8NBP7
• Chromosome Location: 1p34.1-p32

Citation

MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells.

Journal: FEBS letters    PubMed ID: 25613181    Data: 2015/4/14

Authors: Xiang Wang, Evan Berry, Carlos Fernandez-Patron

Article Snippet:To overexpress MMP-2, the cells were transduced with AdMMP-2 or control AdGFP (3 pfu/cell).To overexpress MMP-2, the cells were transduced with AdMMP-2 or control AdGFP (3 pfu/cell).. To study the effects of MMP-2 overexpression on PCSK9-induced LDLR degradation, the cells transduced with AdMMP-2 or AdGFP were incubated in aMEM with 1% bovine serum albumin overnight before adding recombinant human (rh) PCSK9 (Creative BioMart, NY, USA) or vehicle.. To study whether MMP-2 catalytic activity was required for protection, the cells were incubated with the 40 lmol/L MMP-2 inhibitor III (EMD Millipore) for 1 h before adding rhPCSK9.To study whether MMP-2 catalytic activity was required for protection, the cells were incubated with the 40 lmol/L MMP-2 inhibitor III (EMD Millipore) for 1 h before adding rhPCSK9.