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Recombinant Human OSM

Cat.No.: OSM-513H
Product Overview: Recombinant Human Oncostatin M/ OSM Protein, HEK293 Expressed (rhOSM), Ala26-Arg252 (Accession# NP_065391.1 ) was expressed in human 293 cells (HEK293).
Description: Oncostatin M, also known as OSM, is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Of these cytokines it most closely resembles leukemia inhibitory factor (LIF) in both structure and function. However, it is as yet poorly defined and is proving important in liver development, haematopoeisis, inflammation and possibly CNS development. It is also associated with bone formation and destruction. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR. Oncostatin M (OSM) was previoustly identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6 and G-CSF, has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degree but is not stable at 90 degrees. The three dimensional structure of human OSM has been solved to atomic resolution, confirming the predicted long chain four helix bundle topology. Comparing this structure with the known structures of other known LC cytokines shows it to be most closely related to LIF.
Source: HEK293
Species: Human
Predicted N Terminal: Ala26
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity: The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). The ED50 for this effect is typically 2-10 ng/mL.
Molecular Mass: rhOSM contains no "tag" and has a calculated MW of 25.8 kDa. DTT-reduced protein migrates as 30 kDa protein due to glycosylation.
Endotoxin: Less than 1.0 EU endotoxin per μg rhOSM by the LAL method.
Purity: >95% purity as determined by SDS-PAGE of reduced rhOSM.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade-8 centigrade); After reconstitution under sterile conditions for 1 month (4 centigrade-8 centigrade) or 3 months (-20 centigrade to -70 centigrade).
Gene Name: OSM oncostatin M [ Homo sapiens ]
Official Symbol: OSM
Synonyms: OSM; oncostatin M; oncostatin-M; MGC20461;
Gene ID: 5008
mRNA Refseq: NM_020530
Protein Refseq: NP_065391
MIM: 165095
UniProt ID: P13725
Chromosome Location: 22q12.2
Pathway: Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
Function: cytokine activity; growth factor activity; oncostatin-M receptor binding;

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