| Description : |
Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, which also includes IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and shares a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor β or LIF Receptor β to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 10 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1 × 10^5 units/mg. |
| Molecular Mass : |
23.8 kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
210 |
| AA Sequence : |
MAAIGSCSKEYRVLLGQLQKQTDLM QDTSRLLDPYIRIQGLDVPKLREHC RERPGAFPSEETLRGLGRRGFLQTL NATLGCVLHRLADLEQRLPKAQDLE RSGLNIEDLEKLQMARPNILGLRNN IYCMAQLLDNSDTAEPTKAGRGASQ PPTPTPASDAFQRKLEGCRFLHGYH RFMHSVGRVFSKWGESPNRSRRHSP HQALRKGVRR |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant human Oncostatin M(209 a.a.) (rhOSM) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhOSM should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
