"F2" Related Products

Native Pig F2

Cat.No.: F2-647P
Product Overview: Thrombin is from Procine blood.
Description: Thrombin is a "trypsin-like" serine protease protein. Prothrombin (coagulation factor II) is proteolytically cleaved to form thrombin in the coagulation cascade, which ultimately results in the stemming of blood loss. As a result of cleaving, thrombin has two chains: a light chain (A chain, MW ~6 kDA) and a heavy chain (B chain, MW ~31 kDa). These two chains are joined by one disulfide bond. Functions of thrombin are that it converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzes many other coagulation-related reactions. Thrombin also contains g-carboxyglutamyl residues. These modified glutamyl residues are the result of carboxylation by a microsomal enzyme, vitamin Kdependent carboxylase. g-Carboxyglutamyl residues are necessary for the Ca2+-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
Source: Procine Blood
Species: Procine
Form: Lyophilized Powder from 20 mM PBS, pH 7.4 containing 0.9% NaCl.
Purity: >95% by SDS-PAGE and HPLC analyses.
Optimum pH: Stable at neutral pH
Notes: 1. Avoid keeping with heavy metal; 2. Recommended to aliquot the solutions in plastic tubes.Optimal Cleavage Sites1. A-B-Pro-Arg-||-X-Y where A and B are hydrophobic amino acids and X and Y are nonacidic amino acids 2. Gly-Arg-||-Gly
Storage: Two year when stored at -20 centigrade and stable at room temperature for 3 weeks. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile water to a concentration of 10-100 u/ml. Stock solutions should be apportioned into working aliquots and stored at -20°C.
Gene Name: F2 coagulation factor II (thrombin) [ Sus scrofa ]
Official Symbol: F2
Synonyms: prothrombin; thrombin
Gene ID: 100144442
mRNA Refseq: NM_001122985
Protein Refseq: NP_001116457
UniProt ID: Q19AZ8
Chromosome Location: 2; 2
Pathway: Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Neuroactive ligand-receptor interaction, conserved biosystem
Function: calcium ion binding; serine-type endopeptidase activity

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