Active Recombinant Mouse F2 protein(Met1-Gly618), His-tagged

• Cat.No.: F2-1186M
• Product Overview: Recombinant Mouse F2 protein(NP_034298.1)(Met1-Gly618), fused with C-terminal His tag, was expressed in HEK293.

Specification

• similar: F2
• Alias: F2; coagulation factor II; prothrombin; Cf2; FII; Cf-2; thrombin
• Source: HEK293
• Species: Mouse
• Conjugate: C-His
• Tag: His
• Protein length: Met1-Gly618
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• MolecularMass: The secreted recombinant mouse F2 consists of 605 amino acids (pro form) and has a calculated molecular mass of 69.3 kDa. The apparent molecular mass of the recombinant mouse F2 is approximately 85 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• GeneName: F2 coagulation factor II [ Mus musculus ]
• GeneNameWeblink: 14061
• OfficialSymbol: F2
• OfficialSymbolWeblink: 14061
• GeneIDWeblink: 14061
• mRNARefseq: NM_010168
• mRNARefseqWeblink: NM_010168.2
• ProteinRefseqWeblink: NP_034298.1
• Synonyms: F2; coagulation factor II; prothrombin; Cf2; FII; Cf-2; thrombin
• Gene ID: 14061

Gene Information

• Synonyms: F2; coagulation factor II; prothrombin; Cf2; FII; Cf-2; thrombin
• Gene ID: 14061

Reviews

01/01/2022

Quality control is evident. This product ensures accuracy in our studies.

01/27/2019

Our lab swears by this protein. Fast shipping is a definite bonus.

12/18/2018

Consistently reliable. The shipping speed is appreciated in our fast-paced lab.

Q&As

Can you discuss the role of the F2 gene in the synthesis of vitamin K-dependent clotting factors, and how disruptions in this process may lead to coagulation disorders?

The F2 gene plays a crucial role in the synthesis of vitamin K-dependent clotting factors, including prothrombin. Disruptions in this process, often due to genetic or nutritional factors, can contribute to coagulation disorders, impacting hemostasis and blood clotting.

How is the F2 gene regulated at the transcriptional level, and what factors influence prothrombin synthesis in hepatocytes?

Transcriptional regulation of the F2 gene involves factors like vitamin K-dependent gamma-carboxylation. Hepatocytes synthesize prothrombin under the influence of vitamin K and other coagulation factors.

The F2 gene encodes prothrombin, a crucial factor in blood coagulation. Prothrombin is converted to thrombin, a key enzyme in the clotting cascade, facilitating fibrin formation.

The F2 gene encodes prothrombin, a crucial factor in blood coagulation. Prothrombin is converted to thrombin, a key enzyme in the clotting cascade, facilitating fibrin formation.

What are the implications of genetic variations in the F2 gene, particularly polymorphisms, on prothrombin function and susceptibility to thrombotic or bleeding disorders?

Genetic variations in F2, including polymorphisms, can influence prothrombin function. Certain variants may predispose individuals to thrombotic or bleeding disorders.

How does genetic variation in the F2 gene impact prothrombin levels, and what are the consequences for an individual's risk of thrombotic events?

Genetic variations in the F2 gene can influence prothrombin levels, affecting an individual's propensity for thrombotic events. Certain variants may predispose individuals to a higher or lower risk of abnormal blood clot formation.