||Recombinant Human Acyl-Coenzyme A Dehydrogenase 8 produced inE.Coliis a non-glycosylated, polypeptide chain containing amino acids 1-415 and having a total molecular mass of 47.7 kda. ACAD8 contains T7 tag at the N-terminus. ACAD-8 is purified by proprietary chromatographic techniques.
||Acyl CoA dehydrogenase is the enzyme used to catalyze the first step of β-oxidation in Fatty acid metabolism. Acyl-coenzyme A (CoA) dehydrogenases (ACADs) are a family of mitochondrial enzymes that catalyze the first dehydrogenation step in the bets-oxidation of fatty acyl-CoA derivatives. Several human ACADs exist and all ACADs catalyze the same initial dehydrogenation of the substrate at the beta-carbon atom and require electron transfer flavoprotein as an alectron acceptor. The predicted 415-amino acid ACAD8 protein contains many of the residues conserved in most other ACADs, including an active site glutamic acid residue and residues important for tetramer formation.
||Sterile Filtered clear solution.
||Greater than 95.0% as determined by SDS-PAGE.
||Acyl-Coenzyme A Dehydrogenase 8 at a concentration of 0.1 mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3, 10 mM DTT.
||• ELISA • MS • Inhibition Assays • Western Blotting
||ACAD8 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.