Recombinant Human Heat Shock 27 kDa Protein 1 HSPB1-320H

Recombinant Human Heat Shock 27 kDa Protein 1

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Recombinant Human Heat Shock 27 kDa Protein 1

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Cat. No. : HSPB1-320H
Product Overview : Recombinant human Hsp27 was overexpressed in E. coli and purified by conventional chromatography techniques (205 aa, 22.7 kDa).
Description : Hsp27 (also known as the Estrogen-Regulated 24K protein and hsp28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38).
Source : E.coli.
Formulation : Liquid. In 20 mM Hepesl buffer (pH 7.5) containing 100 mM KCl, 1 mM DTT.
Molecular Weight : 22.7 kDa (205aa).
Purity : > 95% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Endotoxin Level : < 1.0 EU per 1 μg of protein (determined by LAL method).
Sequence : MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : HSPB1
Synonyms : HSPB1; heat shock 27kDa protein 1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; DKFZp586P1322; HSPB1; OTTHUMP00000024846; heat shock protein beta-1; HSPB1 heat shock 27kDa protein 1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; Stress-responsive protein 27; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1.
Gene ID : 3315
mRNA Refseq : NM_001540.3
Protein Refseq : NP_001531.1
MIM : 602195
UniProt ID : P04792
Chromosome Location : 7q11.23
Function : identical protein binding.

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