"FASLG" Related Products

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Recombinant Human FASLG protein, His-tagged

Cat.No.: FASLG-46H
Product Overview: Recombinant Human FASLG (Pro134-Leu281) fussed with His tag at N-terminal was expressed in HEK293 cells.
Description: Fas ligand also known as FasL, CD178, CD95L, or TNFSF6, is a homotrimeric type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain. Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Apoptosis triggered by Fas-Fas ligand binding plays a fundamental role in the regulation of the immune system. Its functions include:T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lymphoproliferative syndrome (ALPS), a childhood disorder of apoptosis.
Source: HEK293 cells
Species: Human
Tag: His
Predicted N Terminal: His
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity: Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells.The ED50 for this effect is typically 0.1-1.5 ng/mL in the presence of 10 μg/mL of a crosslinking antibody Mouse Anti poly-Histidine Monoclonal Antibody.
Molecular Mass: Has a calculated MW of 17.7 kDa. Protein migrates as 25-32 kDa in reduced SDS-PAGE due to glycosylation.
Endotoxin: Less than 1.0 EU per μg of the rhFasL by the LAL method.
Purity: >95% purity as determined by SDS-PAGE of reduced rhFasL.
Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade-8 centigrade); After reconstitution under sterile conditions for 1 month (4 centigrade-8 centigrade) or 3 months (-20 centigrade to -70 centigrade).
Gene Name: FASLG Fas ligand (TNF superfamily, member 6) [ Homo sapiens ]
Official Symbol: FASLG
Synonyms: APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1; tumor necrosis factor ligand superfamily member 6; CD95 ligand; apoptosis (APO-1) antigen ligand 1; apoptosis antigen ligand; fas antigen ligand
Gene ID: 356
mRNA Refseq: NM_000639
Protein Refseq: NP_000630
MIM: 134638
UniProt ID: P48023
Chromosome Location: 1q23
Pathway: African trypanosomiasis, organism-specific biosystem; Allograft Rejection, organism-specific biosystem; Apoptosis, organism-specific biosystem
Function: cytokine activity; death receptor binding; protein binding

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