Recombinant Human FASLG protein, His-tagged

• Cat.No.: FASLG-1396H
• Product Overview: Recombinant Human FASLG protein(AAH17502.1)(Pro134-Leu281), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Pro134-Leu281
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Fas Ligand, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 75.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 18 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FASLG Fas ligand (TNF superfamily, member 6) [ Homo sapiens ]
• Official Symbol: FASLG
• Synonyms: FASLG; Fas ligand (TNF superfamily, member 6); APT1LG1, TNFSF6, tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6; CD178; FasL; APTL; CD95 ligand; fas antigen ligand; apoptosis antigen ligand; apoptosis (APO-1) antigen ligand 1; tumor necrosis factor (ligand) superfamily, member 6; FASL; CD95L; CD95-L; TNFSF6; APT1LG1
• Gene ID: 356
• mRNA Refseq: NM_000639
• Protein Refseq: NP_000630
• MIM: 134638
• UniProt ID: P48023