| Cat. No. : |
TF-461H |
| Description : |
Human transferrin is a single-chain glycoprotein. It has two potential N-linked glycosylation sites and one potential O-linked glycosylation site. Transferrin binds two Fe3+ ions, with high affinity (Kd ≈ 10−20 M), together with two synergistically bound CO32- ions. The protein is mainly synthesized in the liver and secreted into the plasma. Lower amounts are also produced in other organs, such as the testis and brain. Transferrin is also capable of acting as a cytokine and has additional functions that may not be related to its iron-carrying capacity. Other roles for transferrin include acting as a neurotrophic factor in myotubule formation and as an angiogenic factor in promoting the migration and invasion of endothelial cells. |
| Source : |
human 293 cells. |
| Molecular Mass : |
Under reducing conditions Transferrin migrates as a broad band between 70 and 80 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified Transferrin that has a predicted monomeric molecular mass of 75.2 kDa. |
| PI : |
Transferrin separates into a number of isoforms with a pI between 6.0 and 8.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Transferrin that has a predicted pI of 6.8. |
| % Carbohydrate : |
Transferrin consists of 5-20% carbohydrate by weight. |
| Glycosylation : |
Transferrin contains N- and O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. |
| Activity : |
The activity of Transferrin was measured by its ability to support the growth of HepG2 cells under conditions of reduced serum. Typically 0.1 to 1 g enhances cell proliferation. |
