Recombinant Rat TF cell lysate

Cat.No. : TF-001RCL
Product Overview : Rat TF / Transferrin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.
  • Specification
  • Gene Information
  • Related Products
  • Download
Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Tf transferrin [ Rattus norvegicus ]
Official Symbol : TF
Synonyms : TF; transferrin; serotransferrin; siderophilin; beta-1 metal-binding globulin; liver regeneration-related protein LRRG03; Tfn; Trf; MGC93500;
Gene ID : 24825
mRNA Refseq : NM_001013110
Protein Refseq : NP_001013128
Pathway : Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem;
Function : ferric iron binding; ferric iron transmembrane transporter activity; metal ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
Can TF proteins be used for diagnosing diseases? 03/19/2021

Yes, abnormalities in TF proteins can serve as diagnostic markers for certain diseases.

Are there any approved drugs that target TF proteins? 11/27/2020

Yes, some drugs target TF proteins, especially in the treatment of cancer.

How do TF proteins contribute to autoimmune diseases? 08/25/2020

TF proteins can trigger or suppress immune responses, making them relevant in understanding autoimmune diseases.

How do TF proteins play a crucial role in clinical applications? 03/16/2016

TF proteins are vital for understanding disease mechanisms and can be targeted for therapeutic purposes.

Are there any clinical tests or assays that measure TF protein levels? 03/05/2016

Yes, various assays, like ELISA and Western blot, can be used to quantify TF protein levels.

Customer Reviews (3)

Write a review
Reviews
08/01/2020

    Their team of experts is not only knowledgeable about the TF protein but is also dedicated to assisting researchers like myself in overcoming any challenges that may arise.

    01/22/2018

      This high-quality protein is crucial for the success of my experiments, ensuring that I obtain reliable and meaningful scientific findings.

      04/11/2016

        The TF protein is of exceptional quality, making it a perfect fit for my experimental needs.

        Ask a Question for All TF Products

        Required fields are marked with *

        My Review for All TF Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /
        • Service lnquiry:

        Stay Updated on the Latest Bioscience Trends