| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Description : |
Recombinant Human LD78beta is a 7.8 kda protein containing 70 amino acid residues, which is closely related to MIP-1alpha. LD78b has enhanced receptor binding affinities to CCR5 and the beta-chemokine receptorD6 and has been shown to exhibit activities in HIV supression assays. |
| Amino Acid Sequence : |
APLAADTPTA CCFSYTSRQI PQNFIADYFETSSQCSKPSV IFLTKRGRQV CAD PSEEWVQKYVSDLELSA. |
| Supplied : |
Lyophilized powder with no additives. |
| Purity : |
>98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein. |
| Reactivity : |
Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml. |
| Note : |
If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml. |
| Reconstitution : |
LyophilizedLD78-beta should be reconstituted in water to a concentration of 0.1-1mg/ml. We recommend a quick spin or genlte tap on table top followed by reconstitution in water. Allow to set at least 30 minutes at 4 °C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 °C for up to 1 week or at -20 °C for future use. |
| Storage : |
The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 °C. Reconstituted LD78B should be stored in working aliquots at -20 °C. avoid frequent freeze thaw cycles. |
