Recombinant Human AKT2, His-tagged

• Cat.No.: AKT2-26262TH
• Product Overview: Recombinant fragment corresponding to amino acids 142-481 of Human AKT2 with N-terminal His tag expressed in a Baculovirus infected Sf9 cell expression system; 340 amino acids, MW 41kDa.

Specification

• Description: This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
• Conjugation: HIS
• Tissue specificity: Expressed in all human cell types so far analyzed.
• Form: Liquid
• Storage buffer: Preservative: 150mM ImidazoleConstituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 PH domain.Contains 1 protein kinase domain.

Gene Information

• Gene Name: AKT2 v-akt murine thymoma viral oncogene homolog 2 [ Homo sapiens ]
• Official Symbol: AKT2
• Synonyms: AKT2; v-akt murine thymoma viral oncogene homolog 2; RAC-beta serine/threonine-protein kinase
• Gene ID: 208
• mRNA Refseq: NM_001243027
• Protein Refseq: NP_001229956
• MIM: 164731
• Uniprot ID: P31751
• Chromosome Location: 19q13.1-q13.2
• Pathway: AKT phosphorylates targets in the cytosol, organism-specific biosystem; AKT phosphorylates targets in the nucleus, organism-specific biosystem; AKT-mediated inactivation of FOXO1A, organism-specific biosystem; Activation of PKB, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem
• Function: ATP binding; ATP binding; kinase activity; nucleotide binding; protein binding