| Species : |
Human |
| Source : |
Sf21 Cells |
| Tag : |
Non |
| Description : |
Heparin-binding EGF-like growth factor (HB-EGF) is a monomeric heparin binding O-glycosylated protein and a member of the EGF family of proteins. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF contributes to cell adhesion, invasion, and angiogenesis, which are integral to transcoelomic metastasis in ovarian cancer. |
| Molecular Weight : |
The predicted molecular weight of Recombinant Human HB-EGF is 9.5 kDa. However, this recombinant protein is heterogenously O-glycosylated and the actual molecular weight as observed by migration on SDS Page is 12 kDa. |
| Formulation : |
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present. |
| State Of Matter : |
Lyophilized. |
| Purity : |
>97% by SDS Page and analyzed by silver stain. |
| Endotoxin : |
<1.0 EU/µg as determined by the LAL method. |
| Biological Activity : |
The biological activity of Human HB-EGF was determined by its ability to stimulate proliferation in a mouse fibroblast cell line, Balb/3T3. The expected ED50 for this effect is typically 0.15 - 0.75 ng/mL. The cell number is assessed in a fluorometric assay using the redox sensitive dye, Resazurin. |
| Storage : |
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |
