||Heparin-binding EGF-like growth factor (HB-EGF) is a monomeric heparin binding O-glycosylated protein and a member of the EGF family of proteins. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF contributes to cell adhesion, invasion, and angiogenesis, which are integral to transcoelomic metastasis in ovarian cancer.
||Sf 21 insect cells.
||The predicted molecular weight of Recombinant Human HB-EGF is 9.5 kDa. However, this recombinant protein is heterogenously O-glycosylated and the actual molecular weight as observed by migration on SDS Page is 12 kDa.
||This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
|State Of Matter:
||>97% by SDS Page and analyzed by silver stain.
||<1.0 EU/µg as determined by the LAL method.
||The biological activity of Human HB-EGF was determined by its ability to stimulate proliferation in a mouse fibroblast cell line, Balb/3T3. The expected ED50 for this effect is typically 0.15 - 0.75 ng/mL. The cell number is assessed in a fluorometric assay using the redox sensitive dye, Resazurin.
||This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.