Active Recombinant Human HBEGF protein

Species :	Human
Source :	E.coli
Tag :	Non
Protein Length :	63-148 aa
Form :	Lyophilized from a 0.2 µm filtered concentrated solution in 10 mM PB, 500 mM NaCl, 5% trehalose, pH 7.4.
Bio-activity :	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10^6 U/mg.
Molecular Mass :	Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
AASequence :	DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL
Endotoxin :	Less than 0.1 EU/µg of rHuHB-EGF as determined by LAL method.
Purity :	> 97% by SDS-PAGE and HPLC analyses.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	HBEGF heparin-binding EGF-like growth factor [ Homo sapiens ]
Official Symbol	HBEGF
Synonyms	HBEGF; heparin-binding EGF-like growth factor; diphtheria toxin receptor (heparin binding epidermal growth factor like growth factor) , DTR, DTS, HEGFL; proheparin-binding EGF-like growth factor; Diphtheria toxin receptor (heparin binding EGF like growth factor); heparin binding epidermal growth factor; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); DTR; DTS; DTSF; HEGFL;
Gene ID	1839
mRNA Refseq	NM_001945
Protein Refseq	NP_001936
MIM	126150
UniProt ID	Q99075