Cat. No. : |
Prkaca-608M |
Product Overview : |
Recombinant Mouse Prkaca is produced inE. coli.MW=41kDa. |
Description : |
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the AMP-activated protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
Source : |
E. coli. |
Purity : |
≥90% (SDS-PAGE). |
Formulation : |
Liquid. In 25mM potassium phosphate, pH 6.5, containing 5mM β-mercaptoethanol, 5mM EDTA, 150mM NaCl and 50% glycerol. |
Application : |
In vitro enzymological studies of neural and hormonal signal transduction Phosphorylation of target proteins in vivo (via microinjection) including ion channels, transcriptional activator proteins and regulatory enzymes of glycogen metabolism. |
Reconstitution : |
Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
Specific Activity : |
1.5x107U/mg. |
Storage And Stability : |
-80°C. Stable for at least 6 months after receipt when stored at -20°C. Stable for 2 weeks when stored at +4°C. Avoid freeze/thaw cycles. |