Active Recombinant Full Length Human PRKACA Protein, DDK-tagged, Biotinylated

• Cat.No.: PRKACA-2051HF
• Product Overview: Full-length human PKACα [1-351(end) amino acids of accession number NP_002721.1] was expressed as N-terminal DYKDDDDK tagged, biotinylated protein (44 kDa) using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: N-DDK
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Protein length: 1-351 a.a.
• Purity: 93%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: protein kinase, cAMP-dependent, catalytic, alpha
• Official Symbol: PRKACA
• Synonyms: PRKACA;PKACA;PPNAD4;PKACa
• Protein Refseq: NP_002721.1
• UniProt ID: P17612