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Active Recombinant Mouse Cst3 protein, His-tagged

Cat.No. : Cst3-2205M
Product Overview : Recombinant Mouse Cst3 protein(NP_034106.2)(Met1-Ala140), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from sterile 20mM HEPES, 150mM NaCl, pH 7.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is < 25 nM.
Molecular Mass : The secreted recombinant mouse CST3 consists of 131 amino acids and has a calculated molecular mass of 15 kDa. The recombinant protein migrates as three bands (15, 18 & 21-24 kDa) in SDS-PAGE under reducing conditions.
Proteinlength : Met1-Ala140
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Cst3 cystatin C [ Mus musculus ]
Official Symbol : Cst3
Synonyms : CST3; cystatin C; cystatin-C; cystatin 3; cystatin-3; CysC;
Gene ID : 13010
mRNA Refseq : NM_009976
Protein Refseq : NP_034106

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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