Active Recombinant Human CST3 protein, His-tagged

• Cat.No.: CST3-859H
• Product Overview: Active Recombinant Human CST3 protein(NP_000090.1) (Ser 27-Ala 146) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 27-146 aa
• Form: Lyophilized from sterile 25mM HEPES, 0.15mM NaCl, pH 7.7.
• Bio-activity: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is < 12 nM.
• Molecular Mass: The mature recombinant human Cystatin-C consists of 131 amino acids and predicts a molecular mass of 14.8 kDa. The rh Cystatin-C migrates as approximately 17 kDa band in SDS-PAGE as a result of glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CST3 cystatin C [ Homo sapiens ]
• Official Symbol: CST3
• Synonyms: CST3; cystatin C; cystatin C (amyloid angiopathy and cerebral hemorrhage); cystatin-C; cystatin 3; cystatin-3; gamma-trace; post-gamma-globulin; bA218C14.4 (cystatin C); neuroendocrine basic polypeptide; ARMD11; MGC117328
• Gene ID: 1471
• mRNA Refseq: NM_000099
• Protein Refseq: NP_000090
• MIM: 604312
• UniProt ID: P01034