Myeloperoxidase Chlorination Fluorometric Assay Kit

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Myeloperoxidase Chlorination Fluorometric Assay Kit

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Cat.No. : Kit-0606
Product Overview : Myeloperoxidase Chlorination Fluorometric Assay provides a convenient fluorescence-based method for detecting the MPO chlorination activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the non-fluorescent 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid (APF), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound fluorescein. Fluorescein fluorescence is analyzed with an excitation wavelength of 480-495 nm and an emission wavelength of 515-525 nm. The kit includes a MPO-specific inhibitor for distinguishing MPO activity from MPO-independent fluorescence.
Storage : Stability: 6 months;Storage: 4°C;Remove the Myeloperoxidase Control from the kit and store at -20°C. The rest of the components should be stored at 4°C. This kit will perform as specified if used before the expiration date indicated on the outside of the box.

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