| Cat.No. : |
MPO-51H |
| Product Overview : |
Recombinant Human Myeloperoxidase is produced by our Mammalian expression system and the target gene encoding Ala49-Ser745 is expressed with a 10His tag at the C-terminus. |
| Description : |
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. |
| Source : |
Human Cells |
| Species : |
Human |
| Tag : |
His |
| AA Sequence : |
AAPAVLGEVD TSLVLSSMEE AKQLVDKAYK ERRESIKQRL RSGSASPMEL LSYFKQPVAA TRTAVRAADY LHVALDLLER KLRSLWRRPF NVTDVLTPAQ LNVLSKSSGC AYQDVGVTCP EQDKYRTITG MCNNRRSPTL GASNRAFVRW LPAEYEDGFS LPYGWTPGVK RNGFPVALAR AVSNEIVRFP TDQLTPDQER SLMFMQWGQL LDHDLDFTPE PAARASFVTG VNCETSCVQQ PPCFPLKIPP NDPRIKNQAD CIPFFRSCPA CPGSNITIRN QINALTSFVD ASMVYGSEEP LARNLRNMSN QLGLLAVNQR FQDNGRALLP FDNLHDDPCL LTNRSARIPC FLAGDTRSSE MPELTSMHTL LLREHNRLAT ELKSLNPRWD GERLYQEARK IVGAMVQIIT YRDYLPLVLG PTAMRKYLPT YRSYNDSVDP RIANVFTNAF RYGHTLIQPF MFRLDNRYQP MEPNPRVPLS RVFFASWRVV LEGGIDPILR GLMATPAKLN RQNQIAVDEI RERLFEQVMR IGLDLPALNM QRSRDHGLPG YNAWRRFCGL PQPETVGQLG TVLRNLKLAR KLMEQYGTPN NIDIWMGGVS EPLKRKGRVG PLLACIIGTQ FRKLRDGDRF WWENEGVFSM QQRQALAQIS LPRIICDNTG ITTVSKNNIF MSNSYPRDFV NCSTLPALNL ASWREASHHH HHHHHHH |
| Endotoxin : |
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Purity : |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage : |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer : |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0. |
| Reconstitution : |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| SDS-PAGE : |
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