| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Fc&His |
| Protein Length : |
49-118 aa |
| Form : |
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-activity : |
1.Measured by its ability to stimulate p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells. 0.01-1 ng/mL of Recombinant Human IGF1 can effectively enhance p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.
2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1 μg/mL (100 μL/well) can bind recombinant human IGF1 with a linear range of 30-250 ng/mL. 3.Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 7.5-30 ng/mL, corresponding to a specific activity of 3.33×10^4-1.33×10^5units/mg. |
| Molecular Mass : |
34.44 kDa |
| Endotoxin : |
< 0.1 EU/μg of the protein by LAL method. |
| Purity : |
≥ 85 % as determined by SDS-PAGE. |
| Storage : |
Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
| Reconstitution : |
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
