Active Recombinant Human IGF1 protein(Gly49-Ala118)

• Cat.No.: IGF1-60H
• Product Overview: Recombinant Human IGF1 (P05019-1) (Gly49-Ala118) was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: N/A
• Protein length: Gly49-Ala118
• Form: Lyophilized from sterile 30% Acetonitrile, 0.1% TFA. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human IGF1 at 2 μg/ml (100 μl/well) can bind Human IGFBP4 His, the EC50 of Human IGFBP4 His is 10-60 ng/mL. 2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 3.5-14 ng/mL.
• Molecular Mass: The recombinant human IGF1 consists of 71 amino acids and has a calculated molecular mass of 7.8 KDa.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IGF1 insulin-like growth factor 1 (somatomedin C) [ Homo sapiens ]
• Official Symbol: IGF1
• Synonyms: IGF1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; IGF1A; MGF; IGF-IA; IGF-IB; somatomedin-C; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; IGFI; IGF-I
• Gene ID: 3479
• mRNA Refseq: NM_000618
• Protein Refseq: NP_000609
• MIM: 147440
• UniProt ID: P05019