Species : |
Human |
Source : |
Sf9 Cells |
Tag : |
His |
Protein Length : |
Full length |
Description : |
MYT1 is a member of a family of neural specific, zinc finger-containing DNA-binding proteins which binds to the promoter regions of proteolipid proteins of the central nervous system and plays a role in the developing nervous system. MYT1 of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. The recombinant MYT1 fragments containing either the upstream 2 zinc fingers or the downstream 4 zinc fingers bound the same cis regulatory element in the PLP1 promoter in vitro and DNA binding required Zn (2+), but not other divalent cations. |
Bio-activity : |
The specific activity of MYT1 was determined to be 14 nmol/min/mg as per actity assay protocol. |
Molecular Mass : |
Observed MW: ~65 kDa. Calculated MW: ~58.7 kDa. |
Purity : |
The purity of MYT1 was determined to be > 90 % by densitometry. |
Stability : |
One year at -70 centigrade from date of shipment. |
Storage : |
Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : |
0.1 μg/μL |
Storage Buffer : |
Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol. |
Shipping : |
Dry ice |