Recombinant Active Human MYT1 Protein (Full length), N-His-tagged

• Cat.No.: MYT1-17H
• Product Overview: Recombinant full-length human MYT1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Description: MYT1 is a member of a family of neural specific, zinc finger-containing DNA-binding proteins which binds to the promoter regions of proteolipid proteins of the central nervous system and plays a role in the developing nervous system. MYT1 of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. The recombinant MYT1 fragments containing either the upstream 2 zinc fingers or the downstream 4 zinc fingers bound the same cis regulatory element in the PLP1 promoter in vitro and DNA binding required Zn (2+), but not other divalent cations.
• Source: Sf9 insect cells
• Species: Human
• Tag: N-His
• Bio-activity: The specific activity of MYT1 was determined to be 14 nmol/min/mg as per actity assay protocol.
• Molecular Mass: Observed MW: ~65 kDa.
  Calculated MW: ~58.7 kDa.
• Protein Length: Full length
• Purity: The purity of MYT1 was determined to be > 90 % by densitometry.
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: PKMYT1 protein kinase, membrane associated tyrosine/threonine 1 [ Homo sapiens (human) ]
• Official Symbol: MYT1
• Synonyms: PKMYT1; protein kinase, membrane associated tyrosine/threonine 1; MYT1; PPP1R126; membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; myt1 kinase; protein phosphatase 1, regulatory subunit 126; EC 2.7.11.1
• Gene ID: 9088
• mRNA Refseq: NM_004203
• Protein Refseq: NP_004194
• MIM: 602474
• UniProt ID: Q99640