Recombinant Active Mouse IL12A Protein, His-tagged(C-ter) Il12a-140M

Recombinant Active Mouse IL12A Protein, His-tagged(C-ter)

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Recombinant Active Mouse IL12A Protein, His-tagged(C-ter)

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Cat.No. : Il12a-140M Optional Service: Optional requirements on this protein
Product Overview : Recombinant Active Mouse IL12A Protein with His tag (C-ter) was expressed in E. coli.
Description : This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
Source : E. coli
Species : Mouse
Tag : His(C-ter)
Form : Powder
Bio-activity : Determined by its ability to induce proliferation in T-cell enriched PBMC. The ED50 for this effect is < 0.2 ng/mL.
AA Sequence : MRVIPVSGPA RCLSQSRNLL KTTDDMVKTA REKLKHYSCT AEDIDHEDIT RDQTSTLKTC LPLELHKNES CLATRETSST TRGSCLPPQK TSLMMTLCLG SIYEDLKMYQ TEFQAINAAL QNHNHQQIIL DKGMLVAIDE LMQSLNHNGE TLRQKPPVGE ADPYRVKMKL CILLHAFSTR VVTINRVMGY LSSA
Endotoxin : Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
Purity : > 98% (by SDS-PAGE)
Applications : SDS-PAGE
Notes : For laboratory research only, not for drug, diagnostic or other use.
Storage : Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Storage Buffer : PBS (pH 7.4)
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
SDS-PAGE :

Gene Name : Il12a interleukin 12a [ Mus musculus ]
Official Symbol : Il12a
Synonyms : IL12A; interleukin 12a; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232;
Gene ID : 16159
mRNA Refseq : NM_001159424
Protein Refseq : NP_001152896
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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