Active Recombinant Mouse Il12a Protein

• Cat.No.: Il12a-086M
• Product Overview: Purified recombinant protein of Mouse interleukin 12a (Il12a), transcript variant 2 without tag was expressed in CHO cell.

Specification

• Description: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
• Source: CHO
• Species: Mouse
• Bio-activity: Assay #1: ED50 was determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is > 0.1 ng/ml, corresponding to a specific activity of > 1 x 10^7 units/mg. Assay #2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is <0.1ng/ml, corresponding to a specific activity of >1 x 10^7 units/mg.
• Molecular Mass: 75 kDa
• AA Sequence: p35 Subunit: RVIPVSGPARCLSQSRNLLKTTDDM VKTAREKLKHYSCTAEDIDHEDITR DQTSTLKTCLPLELHKNESCLATRE TSSTTRGSCLPPQKTSLMMTLCLGS IYEDLKMYQTEFQAINAALQNHNHQ QIILDKGMLVAIDELMQSLNHNGET LRQKPPVGEADPYRVKMKLCILLHA FSTRVVTINRVMGYLSSA
  p40 Subunit: MWELEKDVYVVEVDWTPDAPGETVN LTCDTPEEDDITWTSDQRHGVIGSG KTLTITVKEFLDAGQYTCHKGGETL SHSHLLLHKKENGIWSTEILKNFKN KTFLKCEAPNYSGRFTCSWLVQRNM DLKFNIKSSSSSPDSRAVTCGMASL SAEKVTLDQRDYEKYSVSCQEDVTC PTAEETLPIELALEARQQNKYENYS TSFFIRDIIKPDPPKNLQMKPLKNS QVEVSWEYPDSWSTPHSYFSLKFFV RIQRKKEKMKETEEGCNQKGAFLVE KTSTEVQCKGGNVCVQAQDRYYNSS CSKWACVPCRVRS
• Endotoxin: Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
• Purity: >95%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Storage Buffer: Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2
• Reconstitution: Resuspend the protein in the desired concentration in proper buffer.

Gene Information

• Gene Name: Il12a interleukin 12a [ Mus musculus (house mouse) ]
• Official Symbol: Il12a
• Synonyms: Il12a; interleukin 12a; p35; Ll12a; Il-12a; IL-12p35; interleukin-12 subunit alpha; CLMF p35; IL-12 p35 subunit; IL-12 subunit p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12a p35 subunit; interleukin-12 p35 subunit
• Gene ID: 16159
• mRNA Refseq: NM_008351
• Protein Refseq: NP_032377
• UniProt ID: P43431

Reviews

01/14/2021

Suitable for co-immunoprecipitation.

12/21/2020

Consistent in bioactivity.

11/16/2020

Good for receptor binding studies.

Q&As

How do mass spectrometry-based techniques reveal post-translational modifications on IL12A?

Mass spectrometry identifies IL12A's post-translational modifications and their roles.

What strategies validate IL12A's role in immune response modulation using knockout models?

Knockout models validate IL12A's roles in immune response modulation.

How do protein footprinting assays unveil IL12A's binding interfaces with receptor partners?

Protein footprinting assays reveal IL12A's binding interfaces with receptors.

What experimental methods quantify IL12A's secretion kinetics and stability in cell culture?

ELISA and stability assays quantify IL12A secretion and stability in culture.

Can you discuss the use of SPR in characterizing IL12A's interactions with antibodies and inhibitors?

SPR characterizes IL12A's interactions with antibodies and inhibitors.

What cellular assays assess IL12A's ability to induce cytokine production in immune cells?

Cellular assays gauge IL12A's cytokine induction in immune cells.

How does isothermal titration calorimetry provide insights into IL12A's binding affinity with receptors?

Isothermal titration calorimetry measures IL12A's binding affinity with receptors.

What techniques evaluate the impact of IL12A genetic variants on protein structure and function?

Structural biology techniques analyze IL12A genetic variant impacts.

Could you elaborate on the use of site-directed mutagenesis in probing IL12A's functional domains?

Site-directed mutagenesis probes IL12A's functional domains and interactions.

How does in vivo bioluminescence imaging track IL12A expression dynamics in living organisms?

Bioluminescence imaging tracks IL12A expression dynamics in vivo.