Recombinant Human IL12A cell lysate

Cat.No. : IL12A-2435HCL
Product Overview : Human IL12A / NKSF1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL12A interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) [ Homo sapiens ]
Official Symbol : IL12A
Synonyms : IL12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); NKSF1; interleukin-12 subunit alpha; CLMF; cytotoxic lymphocyte maturation factor 1; p35; IL 12; subunit p35; IL 12A; IL35 subunit; interleukin 12; interleukin 12 alpha chain; natural killer cell stimulatory factor 1; 35 kD subunit; NF cell stimulatory factor chain 1; NFSK; CLMF p35; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; natural killer cell stimulatory factor 1, 35 kD subunit; P35; IL-12A;
Gene ID : 3592
mRNA Refseq : NM_000882
Protein Refseq : NP_000873
MIM : 161560
UniProt ID : P29459
Chromosome Location : 3p12-q13.2
Pathway : African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem;
Function : contributes_to cytokine activity; contributes_to cytokine activity; contributes_to growth factor activity; contributes_to growth factor activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; protein binding; protein heterodimerization activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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01/14/2021

    Suitable for co-immunoprecipitation.

    12/21/2020

      Consistent in bioactivity.

      11/16/2020

        Good for receptor binding studies.

        Q&As (10)

        Ask a question
        How do mass spectrometry-based techniques reveal post-translational modifications on IL12A? 08/18/2022

        Mass spectrometry identifies IL12A's post-translational modifications and their roles.

        What strategies validate IL12A's role in immune response modulation using knockout models? 06/12/2022

        Knockout models validate IL12A's roles in immune response modulation.

        How do protein footprinting assays unveil IL12A's binding interfaces with receptor partners? 06/26/2021

        Protein footprinting assays reveal IL12A's binding interfaces with receptors.

        What experimental methods quantify IL12A's secretion kinetics and stability in cell culture? 12/17/2020

        ELISA and stability assays quantify IL12A secretion and stability in culture.

        Can you discuss the use of SPR in characterizing IL12A's interactions with antibodies and inhibitors? 10/25/2020

        SPR characterizes IL12A's interactions with antibodies and inhibitors.

        What cellular assays assess IL12A's ability to induce cytokine production in immune cells? 05/19/2019

        Cellular assays gauge IL12A's cytokine induction in immune cells.

        How does isothermal titration calorimetry provide insights into IL12A's binding affinity with receptors? 06/05/2018

        Isothermal titration calorimetry measures IL12A's binding affinity with receptors.

        What techniques evaluate the impact of IL12A genetic variants on protein structure and function? 03/08/2018

        Structural biology techniques analyze IL12A genetic variant impacts.

        Could you elaborate on the use of site-directed mutagenesis in probing IL12A's functional domains? 11/23/2017

        Site-directed mutagenesis probes IL12A's functional domains and interactions.

        How does in vivo bioluminescence imaging track IL12A expression dynamics in living organisms? 02/16/2017

        Bioluminescence imaging tracks IL12A expression dynamics in vivo.

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