| Description : |
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008] |
| Source : |
E. coli |
| Species : |
Mouse |
| Tag : |
His(C-ter) |
| Form : |
Powder |
| Bio-activity : |
Determined by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is < 5 pg/ml. The specific activity of recombinant mouse IL-1 alpha is > 2 x 10^8 IU/mg. |
| AA Sequence : |
MSAPYTYQSDLRYKLMKLVRQKFVM NDSLNQTIYQDVDKHYLSTTWLNDL QQEVKFDMYAYSSGGDDSKYPVTLK ISDSQLFVSAQGEDQPVLLKELPET PKLITGSETDLIFFWKSINSKNYFT SAAYPELFIATKEQSRVHLARGLPS MTDFQIS |
| Endotoxin : |
Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test. |
| Purity : |
> 95% (by SDS-PAGE) |
| Applications : |
SDS-PAGE |
| Notes : |
For laboratory research only, not for drug, diagnostic or other use. |
| Storage : |
Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Storage Buffer : |
PBS (pH 7.4) |
| Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
