Active Recombinant Human IL1A protein

• Cat.No.: IL1A-501H
• Product Overview: Active Recombinant Human IL1A protein(NP_000566.3) (Ser 113-Ala 271) was expressed and purified in Human Cells.

Specification

• Species: Human
• Source: Human Cells
• Tag: Non
• Protein Length: 113-271 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0.
• Bio-activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 0.2-1 ng/mL.
• Molecular Mass: The recombinant human IL1α consists of 159 amino acids and has a predicted molecular mass of 18 kDa. As a result of glycosylation, the apparent molecular mass of rhIL1α is approximately 22 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL1A interleukin 1, alpha [ Homo sapiens ]
• Official Symbol: IL1A
• Synonyms: IL1A; interleukin 1, alpha; IL1; interleukin-1 alpha; hematopoietin 1; IL 1A; IL1 ALPHA; IL1F1; preinterleukin 1 alpha; pro interleukin 1 alpha; IL-1 alpha; hematopoietin-1; pro-interleukin-1-alpha; IL-1A; IL1-ALPHA
• Gene ID: 3552
• mRNA Refseq: NM_000575
• Protein Refseq: NP_000566
• MIM: 147760
• UniProt ID: P01583