Active Recombinant Canine IL17A Protein, His-tagged

• Cat.No.: IL17A-12C
• Product Overview: Recombinant canine IL-17A (29-115aa, 133 aa), fused to His tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: IL-17A, also known as CTLA8, is a member of the IL-17 family of cytokines. This family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. The IL-17 family of cytokines includes six members, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F. IL-17A main funcion is stimulates production of IL-6, prostaglandin E and nitric oxide. And other inflammatory cytokines(TNF-alpha, IL-1 beta and IFN -gamma) leads to up-regulation of gene expression and progression and amplification of local inflammation. In addition, it serves induction of co-stimulatory molecules such as ICAM-1 and other cytokines, thereby supporting T cell activation. IL-17 expression has been associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
• Source: HEK293
• Species: Canine
• Tag: His
• Form: Liquid
• Bio-activity: The activity is determined by the IL-6 ELISA in a using NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 10 ng/mL.
• Molecular Mass: 15.6 kDa
• Protein length: 29-115
• AA Sequence: FPQNPGCRNTEDKNFPQHVKVNLNI LNRNTNSRRPSDYYNRSTSPWNLHR NEDPERYPSVIWEAKCRHLGCVNNE GNINYHMNSVPIQQEILVLRRESQH CPHSFRLEKMLVAVGCTCVTPIVRH VA
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at 2 to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: IL17A interleukin 17A [ Canis lupus familiaris (dog) ]
• Official Symbol: IL17A
• Synonyms: IL17A; interleukin 17A; interleukin-17A
• Gene ID: 481837
• mRNA Refseq: NM_001165878
• Protein Refseq: NP_001159350
• UniProt ID: C6L8D7