Active Recombinant Mouse IL17A Protein, His-tagged

• Cat.No.: IL17A-381M
• Product Overview: Recombinant Mouse IL17A Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ala 158 (Accession # Q62386-1). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 18 kDa, 20-23 kDa and 26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Recombinant Mouse IL17A Protein, His-tagged at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 8-128 ng/mL (QC tested).
  Recombinant Mouse IL17A Protein, His-tagged stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 2.132-5.005 ng/ml.
• Molecular Mass: 15.8 kDa
• Protein length: Ala 26 - Ala 158
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Il17a interleukin 17A [ Mus musculus ]
• Official Symbol: IL17A
• Synonyms: IL17A; interleukin 17A; interleukin-17A; Il17; Ctla8; IL-17; Ctla-8; IL-17A
• Gene ID: 16171
• mRNA Refseq: NM_010552
• Protein Refseq: NP_034682