Recombinant Cynomolgus ACE2 protein, His-Avi-tagged, Biotinylated

• Cat.No.: ACE2-109CB
• Product Overview: Biotinylated Recombinant Cynomolgus ACE2 protein(A0A2K5X283)(Gln18-Val739), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gln18-Val739
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized SARS-COV-2 Spike RBD, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus ACE2, His Tag with the EC50 of 0.28ug/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.