Species : |
Paguma larvata |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Gln 18-Thr 740 |
Form : |
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant. |
Bio-activity : |
Immobilized Paguma larvata ACE2, His Tag at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S1 protein, Fc Tag with a linear range of 0.156-5 μg/mL.Immobilized Paguma larvata ACE2, His Tag at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD, Fc Tag with a linear range of 0.078-5 μg/mL. |
Molecular Mass : |
This protein carries a polyhistidine tag at the N-terminus.The protein has a calculated MW of 85.7 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |